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KMID : 0613820050150040607
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Journal of Life Science
2005 Volume.15 No. 4 p.607 ~ p.612
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The Inducible form of Heat Shock Protein 70 (Hsp70) is Expressed in the Rat Cerebellar Synapses in Normal Condition
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Cho Sun-Jung
Jung Jae-Seob
Jin Ingn-Yol
Jeong Seung-Hyun
Park In-Sik
Moon Il-Soo
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Abstract
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Heat shock protein 70 (HSP70) is a multigene family composed of constitutively expressed members (Hsc70) and stress-inducible members (Hsp70). In the mammalian nervous system, a considerable amount of HSPs is also synthesized under normal conditions suggesting that they play an important role in the metabolism of unstressed cells. In this study we examined the expression of Hsp70 in the synapses of rat cerebellar neurons. Immunohistochemistry using specific antibodies revealed that both Hsp70 and Hsc70 are expressed in the cerebellar tissue, with strongest expression in Purkinje cells followed by granule cells. Neurons in deep cerebellar nuclei were also intensely stained by Hsp70 antibody. Immunocytochemical stainings of cultured cerebellar cells showed that Hsp70 is expressed in both Purkinje and granule cells. The expression was punctate in the soma and along dendritic trees, and the punctae were colocalized with those of PSD95, a postsynaptic marker. Immunoblotting also indicates that Hsp70 is associated with the postsynaptic density fraction. Taken together, our results indicate that the Hsp70 is expressed in cerebellar neurons in normal conditions, and that some are localized in the synapses
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